Typing and subtyping of influenza viruses and respiratory syncytial viruses by multiplex RT-PCR
نویسندگان
چکیده
منابع مشابه
Molecular detection and subtyping of human influenza A viruses based on multiplex RT-PCR assay
Influenza virus infections have been causing major public health concerns worldwide. Therefore, rapid and accurate diagnostic methods for typing and subtyping of influenza virus are crucial both for patient management and to limit dissemination. In this study, multiplex RT-PCR was developed, optimized and evaluated for detection, typing and subtyping of influenza viruses. The typing assay consi...
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A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus (HRSV). Detection of each viral product and of an internal control was based on determination of specific melting temperatures by the LightCycler software. The lower limit of detection in the multiplex PCR assay was found to be 50 copie...
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*Corresponding author: Fax: +81-82-252-8642, E-mail: takao@ urban.ne.jp In Japan, prefectural and municipal public health institutes are primarily responsible for isolating influenza viruses from clinical specimens. They determine the viruses’ hemagglutinin (HA) subtypes by means of hemagglutinin inhibition (HI) test, but usually not neuraminidase (NA) subtypes because NA subtype-specific antis...
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Type A and B influenza viruses can cause a wide spectrum of illness, and these viruses are responsible for considerable mortality and morbidity. Rapid typing of isolates is desirable when amantadine treatment or prophylaxis of contacts of type A influenza virus carriers is considered, but the available rapid techniques lack sensitivity and standard diagnostic methods require expansion of virus ...
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Laboratory diagnosis of viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA viruses. A rapid real-time multiplex PCR assay was developed for the detection of influenza A and influenza B viruses, human respiratory sy...
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ژورنال
عنوان ژورنال: International Congress Series
سال: 2004
ISSN: 0531-5131
DOI: 10.1016/j.ics.2004.02.057